Bioinformatics Mcqs

Page No. 16

Which of the following is untrue about PRRN?


aPRRN is a web-based program that uses a double nested iterative strategy for multiple alignment


b It performs multiple alignments through two sets of iterations: inner iteration and outer iteration


cIn the outer iteration, an initial random alignment is generated that is used to derive a UPGMA tree


dIn the inner iteration, the sequences are randomly divided into multiple groups



Which of the following is untrue about iterative approach?


aThe iterative approach is based on the idea that an optimal solution can be found by repeatedly modifying existing suboptimal solutions


bBecause the order of the sequences used for alignment is different in each iteration


c This method is also heuristic in nature and does not have guarantees for finding the optimal alignment


dThis method is not based on heuristic methods



Which of the following is untrue regarding T-Coffee?


a It stands for Tree-based Consistency Objective Function for alignment Evaluation


bIt performs progressive sequence alignments as in Clustal.


c The global pair wise alignment is not performed using the Clustal program.


dThe local pair wise alignment is generated by the Lalign program, from which the top ten scored alignments are selected



Which of the following is not a drawback of the progressive alignment method?


aThe progressive alignment method is not suitable for comparing sequences of different lengths because it is a global alignment–based method


b In this method the use of affine gap penalties, long gaps are not allowed, and, in some cases, this may limit the accuracy of the method


cIn this method the use of affine gap penalties, long gaps is allowed, and, in some cases, this may limit the accuracy of the method


dThe final alignment result is also influenced by the order of sequence addition



Clustal is a progressive multiple alignment program available either as a stand-alone or on-line program


a True


bFalse


cether true and false


dnone of these


View Answer True

Which of the following is untrue regarding the Progressive Alignment Method?


aProgressive alignment depends on the stepwise assembly of multiple alignments and is heuristic in nature


bIt speeds up the alignment of multiple sequences through a multistep process


cIt first conducts pair wise alignments for each possible pair of sequences using the Needleman–Wunsch global alignment method and records these similarity scores from the pair wise compariso


d Its drawback is it slows down the alignment of multiple sequences through a single step process



There are two types matrices involved in the study- score matrices and trace matrices.


a True


bFalse


ceither true and false


dnone of these


View Answer True

Which of the following is untrue about Needleman-Wunsch algorithm?


aIt is an example of dynamic programming


b Basic idea here is to build up the best alignment by using optimal alignments of larger subsequences


cIt was first used by Saul Needleman and Christian Wunsch


dIt was first used in 1970



The number of possible global alignments between two sequences of length N is _____


a2NπN√


b22NπN√22NπN√


c2(N−1)πN√


d22NN√


View Answer 22NπN√

In Needleman-Wunsch algorithm, the gaps are scored -2.


a True


bFalse


ceither true and false


dnone of these


View Answer False

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