Bioinformatics Mcqs

Page No. 15

There are two approaches viz. exhaustive and heuristic approaches used in multiple sequence alignment.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

Given a multiple alignment of three sequences, the sum of scores is calculated as the sum of the dissimilarity scores of every pair of sequences at each position.


aTrue


bFalse


ceither true and false


dnone of these


View Answer False

Which of the following scores are not considered while calculating the SP scores?


aAll possible pair wise matches


b All possible mismatches


cAll possible gap costs


d Number of gap penalties



The scoring function for multiple sequence alignment is based on the concept of sum of pairs (SP).


a True


bFalse


ceither true and false


dnone of these


View Answer True

Which of the following cannot be related to multiple sequence alignment?


a Many conserved and functionally critical amino acid residues can be identified in a protein multiple alignment


bMultiple sequence alignment is also an essential prerequisite to carrying out phylogenetic analysis of sequence families and prediction of protein secondary and tertiary structures


cMultiple sequence alignment also has applications in designing degenerate polymerase chain reaction (PCR) primers based on multiple related sequences


dThis method does not contribute much to degenerate polymerase chain reaction (PCR) primers creation



There is a unique advantage of multiple sequence alignment because it reveals more biological information than many pair wise alignments can.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

Related sequences are identified through the database similarity searching and as the process generates multiple matching sequence pairs, it is often necessary to convert the numerous pair wise alignments into a single alignment.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

Match-Box compares segments of some of the nine residues of possible Pair wise alignments


aTrue


bFalse


ceither true and false


dnone of these


View Answer False

. Which of the following is untrue about DIALIGN2?


aIt is a web based program designed to detect local similarities


bIt is designed to detect global similarities


cIt does not apply gap penalties and thus is not sensitive to long gaps


d The method breaks each of the sequences down to smaller segments and performs all possible pair wise alignments between the segments



The major drawback of the progressive and iterative alignment strategies is that they are largely global alignment based and may therefore fail to recognize conserved domains and motifs among highly divergent sequences of varying lengths.


a True


b False


ceither true and false


dnone of these


View Answer True

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