Bioinformatics Mcqs

Page No. 80

Many levels of clone redundancy may be required to build a consensus map because individual clones can have _______


arearrangements


bdeletions


c two separate fragments


dvectors


View Answer vectors

To sequence larger molecules, individual chromosomes are purified and broken into _____ or larger random fragments, which are cloned into vectors designed for large molecules.


a100-Mb


b100-kb


c5000-kb


d600-kb


View Answer 100-kb

When the process is fully automated, a number of priming sites may be used to obtain sequencing results that give optimal separation of bands in each region of the sequence.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer True

The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer True

The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.


a separate, size


bseparate, pH


cassimilate, pH


dassimilate, size


View Answer separate, size

Using ddATP, the resulting synthesis creates a set of nested DNA fragments, each one ending at one of the as in the sequence through the substitution of a fluorescent-labeled ddATP.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer True

Which of the following is untrue about DNA sequencing methods?


aPurified fragments of DNA cut from plasmid/phage clones or amplified by polymerase chain reaction (PCR)


bClones of DNA fragments are denatured to single strands, and one of the strands is hybridized to an oligonucleotide primer


cTaq polymerase is quite heat sensitive


dNew strands of DNA are synthesized from the end of the primer



Granting agency requires a certain level of accuracy in case of errors. Which of the given statements is untrue regarding it?


a In other laboratories, such as those performing a single-attempt sequencing of ESTs, the error rate may be much higher, approximately 1 in 100, including incorrectly identified bases and inserted or deleted bases


bIncorrect bases always translate to the right amino acid


cBase insertions/deletions will cause frame-shifts in the sequence


dMaking alignment with a protein sequence becomes difficult because of frameshifts



NCBI reviews new entries and updates existing ones, as requested.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer True

Investigators are encouraged to submit their newly obtained sequences directly to a member of the International Nucleotide Sequence Database Collaboration, such as the NCBI, DDBJ, and EMBL.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer True

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