Bioinformatics Mcqs

Page No. 53

Which of the following is incorrect regarding Mascot and ProFound?


aProFound is a web server with a set of interconnected programs


b ProFound searches a protein sequence database using MS fingerprinting information


c Bayesian algorithm is not involved in ProFound


dMascot is a web server that identifies proteins based on peptide mass fingerprints, sequence entries, or raw MS/MS data from one or more peptides



ExPASY is a comprehensive proteomics web server with a suite of programs for searching peptide information from the SWISS-PROT and TrEMBL databases.


a True


b False


ceither true and false


dnone of these


View Answer True

Which of the following is incorrect regarding the Protein Identification through Database Searching?


aMS characterization of proteins is highly dependent on bioinformatic analysis


bBioinformatics programs can be used to search for the identity of a protein in a database of theoretically digested proteins


cEven in reality, the protease digestion is always perfect in MS


dThe purpose of the database search is to find exact or nearly exact matches



Electrospray ionization MS and matrix-assisted laser desorption ionization (MALDI) MS only differ in the ionization procedure used.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

Which of the following is incorrect regarding Mass Spectrometry Protein Identification?


aThe proteolysis doesn’t generate a pattern according to molecular weight


bProteins can be identified and characterized using MS


cThe proteins from a two dimensional gel system are first digested in situ with a protease


dProtein spots of interest are excised from the two-dimensional gel



Comparing two-dimensional gel images from various experiments can sometimes pose a challenge because the gels, unlike DNA microarrays, may shrink or warp.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

Which of the following is incorrect regarding 2D-Page?


a It stands for Two-dimensional polyacrylamide gel electrophoresis


bIt separates proteins by charge only


c The gel is run in one direction in a pH gradient under a non-denaturing condition


d It works to separate proteins by isoelectric points (pI)



The classic protein separation methods involve two-dimensional gel electrophoresis followed by gel image analysis.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

ExPASY contains a number of programs to determine posttranslational modifications based on MS molecular mass data.


a True


bFalse


ceither true and false


dnone of these


View Answer True

Cysteine doesn’t make predictions by building profiles.


aTrue


b False


ceither true and false


dnone of these


View Answer False

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