Bioinformatics Mcqs

Page No. 52

which of the following is incorrect about Oligonucleotide Design in A microarray?


aDNA microarrays are generated by fixing oligonucleotides onto a solid support


bThe oligonucleotide array slide represents thousands of preselected genes from an organism


cThe length of oligonucleotides is typically in the range of twenty-five to seventy bases long


dThe oligonucleotides don’t react with cDNA samples



Which of the following is incorrect about a microarray?


aIt is a slide attached with a high-density array of immobilized DNA oligomers representing the entire genome of the species under study


bArray of immobilized DNA oligomers cannot be cDNAs


cEach oligomer is spotted on the slide and serves as a probe for binding to a unique complementary cDNA


dIt is the most commonly used global gene expression profiling method



The PCR amplification step involved in the SAGE procedure means that it requires a large quantity of sample mRNA.


a True


bFalse


ceither true and false


dnone of these


View Answer True

DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels.


a True


bFalse


ceither true and false


dnone of these


View Answer False

Which of the following is untrue about SAGE Genie?


aIt is an NCBI web-based program


b It allows matching of experimentally obtained SAGE tags to known genes


cIt provides an interface for visualizing human gene expression


d It doesn’t filter out linker sequences from experimentally obtained SAGE tags



SAGExProfiler doesn’t provide information about overexpressed or silenced genes.


a True


bFalse


ceither true and false


dnone of these


View Answer False

SAGEmap is a SAGE database created by NCBI.


aTrue


b False


ceither true and false


dnone of these


View Answer True

Which of the following is untrue about the drawbacks of SAGE?


a One or two sequencing errors in the tag sequence can lead to ambiguous or erroneous tag identification


b Correctly sequenced SAGE tag sometimes may correspond to several genes or no gene at all


cCorrectly sequenced SAGE tag always corresponds to several genes


d The drawback with this approach is the sensitivity to sequencing errors



Which of the following is untrue about SAGE?


ahis approach is much more efficient than the EST analysis


b This approach is quite less efficient than the EST analysis


cIt uses a short nucleotide tag to define a gene transcript


d It allows sequencing of multiple tags in a single clone



Which of the following is incorrect regarding Differential In-Gel Electrophoresis?


a Proteins are mixed together before electrophoresis on a two-dimensional gel


bDifferentially expressed proteins in both conditions can’t be visualized in the same gel


cIn this, Differences in protein expression patterns can be detected in a similar way as in fluorescent-labeled DNA microarrays


dProteins from experimental and control samples are labeled with differently colored fluorescent dyes



Goto Page No.

Page 52 of 92

Alphabetical MCQs Categories

Here below You find all kind of categories of MCQs in alphabetical order.


"Comprehensive Collection of MCQs: Alphabetically Organized by Subject"

"Discover an extensive collection of multiple-choice questions (MCQs) neatly categorized by subject from A to Z. This resource is designed for anyone eager to explore various topics in a straightforward and organized manner. Whether you're a student preparing for exams, an educator seeking supplementary materials, or simply curious about different subjects, this compilation offers a convenient way to learn and assess your knowledge. With questions arranged alphabetically, navigation is effortless, allowing you to delve into subjects of interest at your own pace. Engage, learn, and expand your understanding with this accessible and comprehensive repository of MCQs!"


BolPakistan

bolpakistan.com.pk includes Job Mcqs and Pak Mcqs is the Top Largest Mcqs Forum in World, in which you can read Mcqs of All Subjects, PPSC test preparation, FPSC, NTS and PPSC PAST PAPERS, PPSC PAST MCQS. A Collection of Repeated MCQs for JOBs seekers.

Copyright © 2024, Designed & Developed by BolPakistan