Bioinformatics Mcqs

Page No. 50

Which of the following is untrue about LAGAN?


a It stands for Limited Area Global Alignment of Nucleotides

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b It is a web-based program designed for pairwise alignment of small fragments of genomes only

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c It first finds anchors between two genomic sequences using an algorithm that identifies short, exactly matching words

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d Regions that have high density of words are selected as anchors

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Which of the following is untrue about Whole Genome Alignment?


aThis helps to reveal the presence of conserved functional elements

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b It doesn’t help to understand sequence conservation between genomes

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c It be accomplished through direct genome comparison or genome alignment

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dThe alignment at the genome level is fundamentally no different from the basic sequence alignment

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Which of the following is untrue about comparative genomics?


a It is comparison of whole genomes from different organisms

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bIt includes comparison of gene number, gene location, and gene content from these genomes

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c It provides insights into the mechanism of genome evolution and gene transfer among genomes

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d It doesn’t help to reveal the extent of conservation among genomes

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Which of the following is untrue regarding SAGE?


a It stands for Serial analysis of gene expression

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bIt is another high throughput, sequence-based approach for global gene expression profile analysis

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cIt stands for Squared analysis of gene expression

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dUnlike EST sampling, SAGE is more quantitative in determining mRNA expression in a cell

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Which of the following is untrue regarding TIGR Gene Indices?


a It is an EST database that the similar type of clustering method from UniGene

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bIt is an EST database that uses a different clustering method from UniGene

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c It compiles data from dbEST, GenBank mRNA and genomic DNA data, and TIGR’s own

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d None of the mentioned

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Which of the following is untrue regarding UniGene?


aIt is an NCBI EST cluster database

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bOverlapping EST sequences are computationally processed to represent a single expressed gene

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c Each cluster is a set of overlapping EST sequences

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dThe overlapping EST sequences are computationally processed to represent a set of expressed genes

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Which of the following is untrue regarding EST Index Construction?


a The goal of the EST databases is to organize and consolidate the largely redundant EST data

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bThe process includes a preprocessing step that removes masks repeats

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cThere is no screening of vector contaminants

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dThe goal of the EST databases is to improve the quality of the sequence information so the data can be used to extract full-length cDNAs

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GenBank has a special EST database, dbEST that contains EST collections for a large number of organisms.


aTrue

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bFalse

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ceither true and false

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dnone of these

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View Answer True

t has been estimated that up to 11% of cDNA clones may be chimeric.


aTrue

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bFalse

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ceither true and false

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dnone of these

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View Answer True

Which of the following is untrue regarding the drawbacks of expressed sequence tags (ESTs)?


aThey are often of lowquality because they are automatically generated without verification

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b Many bases are ambiguously determined, represented by N’s

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c Frame shift errors and artifactual stop codons are some common errors

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dDespite of all the failures, the translation the sequences is smooth

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