Bioinformatics Mcqs

Page No. 50

Which of the following is untrue about LAGAN?


a It stands for Limited Area Global Alignment of Nucleotides


b It is a web-based program designed for pairwise alignment of small fragments of genomes only


c It first finds anchors between two genomic sequences using an algorithm that identifies short, exactly matching words


d Regions that have high density of words are selected as anchors



Which of the following is untrue about Whole Genome Alignment?


aThis helps to reveal the presence of conserved functional elements


b It doesn’t help to understand sequence conservation between genomes


c It be accomplished through direct genome comparison or genome alignment


dThe alignment at the genome level is fundamentally no different from the basic sequence alignment



Which of the following is untrue about comparative genomics?


a It is comparison of whole genomes from different organisms


bIt includes comparison of gene number, gene location, and gene content from these genomes


c It provides insights into the mechanism of genome evolution and gene transfer among genomes


d It doesn’t help to reveal the extent of conservation among genomes



Which of the following is untrue regarding SAGE?


a It stands for Serial analysis of gene expression


bIt is another high throughput, sequence-based approach for global gene expression profile analysis


cIt stands for Squared analysis of gene expression


dUnlike EST sampling, SAGE is more quantitative in determining mRNA expression in a cell



Which of the following is untrue regarding TIGR Gene Indices?


a It is an EST database that the similar type of clustering method from UniGene


bIt is an EST database that uses a different clustering method from UniGene


c It compiles data from dbEST, GenBank mRNA and genomic DNA data, and TIGR’s own


d None of the mentioned



Which of the following is untrue regarding UniGene?


aIt is an NCBI EST cluster database


bOverlapping EST sequences are computationally processed to represent a single expressed gene


c Each cluster is a set of overlapping EST sequences


dThe overlapping EST sequences are computationally processed to represent a set of expressed genes



Which of the following is untrue regarding EST Index Construction?


a The goal of the EST databases is to organize and consolidate the largely redundant EST data


bThe process includes a preprocessing step that removes masks repeats


cThere is no screening of vector contaminants


dThe goal of the EST databases is to improve the quality of the sequence information so the data can be used to extract full-length cDNAs



GenBank has a special EST database, dbEST that contains EST collections for a large number of organisms.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

t has been estimated that up to 11% of cDNA clones may be chimeric.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

Which of the following is untrue regarding the drawbacks of expressed sequence tags (ESTs)?


aThey are often of lowquality because they are automatically generated without verification


b Many bases are ambiguously determined, represented by N’s


c Frame shift errors and artifactual stop codons are some common errors


dDespite of all the failures, the translation the sequences is smooth



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