Bioinformatics Mcqs

Page No. 48

Which of the following is incorrect about Phrap?


aIt aligns individual fragments in a pairwise fashion using the Smith–Waterman algorithm


bIt doesn’t take input from Phred


cIt is used for sequence assembly


dIt is a UNIX program



Which of the following is incorrect about Phred?


aIt is a UNIX program


b It doesn’t give a probability score in output


cIt is used for base calling


dIt uses a Fourier analysis to resolve fluorescence traces and predict actual peak locations of bases



Which of the following is untrue about base calling and assembly programs?


aThe first step toward genome assembly includes derive base calls


bThe first step toward genome assembly includes assigning associated quality scores


cOne of the steps is to assemble the sequence reads into contiguous sequences


dThere is no identifying overlap between sequence fragments



When a sequence is generated from ____ ends of a single clone, the distance between the two opposing fragments of a clone is fixed to ________ meaning that they are always separated by a distance defined by a _____ length (normally 1,000 to 9,000 bases).


aboth, an uncertain range, clone


bone, an uncertain range, clone


cboth, a certain range, clone


d both, a certain range, gene



Which of the following is incorrect regarding GeneQuiz?


a It is a web server for protein DNA annotation


b It is a web server for protein sequence annotation


c It compares a query sequence against databases using BLAST and FASTA to identify homologs with high similarities


d It performs domain analysis using the PROSITE and Blocks databases



In some circumstances, one mRNA transcript can lead to the translation of more than one protein.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

Which of the following is incorrect regarding genome economy?


a It is a phenomenon of synthesizing more proteins from fewer genes


b This is a major strategy that eukaryotic organisms use to achieve a myriad of genotypic diversities only


cThis is a major strategy that eukaryotic organisms use to achieve a myriad of phenotypic diversities


d There are numerous underlying genetic mechanisms to help account for genome economy



The remote homology detection helps to shed light on the possible functions of the proteins that previously have no functional information at all.


aTrue


bFalse


ceither true and false


dnone of these


View Answer True

Conserved functional sites can be identified by profile and hidden Markov model–based motif and domain search tools such as SMART and InterPro.


a True


bFalse


ceither true and false


dnone of these


View Answer True

Which of the following is incorrect regarding Automated Genome Annotation?


aIt exists because of the need to develop fast and automated methods to annotate the genomic sequences


bThe automated approach relies on homology detection


cThe automated approach doesn’t rely on heuristic sequence similarity searching


dAutomation brings speed in gene annotation process



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