Bioinformatics Mcqs

Page No. 47

Despite the multiple coverage, sometimes certain genomic regions remain unsequenced, mainly owing to cloning difficulties.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer True

The shotgun approach does not require knowledge of physical mapping of the clone fragments, but rather a robust computer assembly program to join the pieces of random fragments into a single, whole-genome sequence.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer True

The shotgun approach _______ sequences clones from _____ of cloned DNA.


arandomly, one end


brandomly, both ends


cspecifically, both ends


dspecifically, one end


View Answer randomly, both ends

In DNA sequencing, the fluorescent traces of the DNA sequences are read by a computer program that assigns bases for each peak in a chromatogram.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer True

Which of the following is untrue about DNA sequencing?


a It is now routinely carried out using the Sanger method


bThis doesn’t make use of DNA polymerases


cThis involves the synthesis of DNA chains of varying length


dThe DNA synthesis is stopped by adding dideoxynucleotides



The _____ resolution genome map is the genomic DNA sequence that can be considered as a type of ______ map describing a genome at the single base-pair level.


ahighest, physical


blowest, physical


chighest, cytological


dlowest, cytological


View Answer highest, physical

Which of the following is incorrect about ARACHNE?


aIt accepts base calls with associated quality scores assigned by Phred as input


b It is a free UNIX program


c It is for the assembly of whole-genome shotgun reads


d It doesn’t involve heuristic approach



TIGR Assembler is a UNIX program from TIGR for assembly of large shotgun sequence fragments.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer True

Which of the following is incorrect about EULER?


aIt is an assembly algorithm


b It uses a Eulerian Superpath approach, which is a polynomial algorithm


cIn this approach, a sequence fragment is broken down to tuples of five nucleotides


dThe tuples are distributed in a diagram with numerous nodes that are all interconnected



VecScreen is a primarily aimed for sequence assembly.


aTrue


bFalse


ceither true and false


dnone of the mentioned


View Answer False

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